Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Load samples and run the gels using your standard protocol. Apr 18, 2016 ethidium bromide is found in pretty much every molecular biology lab around. Ask most biologists about handling it, and youre get a fearful expression and advice to use gloves, etc. These filters use ionexchange resins and activated charcoal to remove the ethidium bromide from. Ethidium bromide is a potent mutagen, often used in laboratories to stain nucleic acids in electrophoresis gels. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required. But a msds for sybr safe reports a ld50 for rats of 5gkg, which is higher. Ethidium bromide laboratory spill and waste handling procedures. Safety data sheet northwest missouri state university. Ethidium bromide solutions and buffer solutions containing ethidium bromide should be filtered, decontaminated, or destroyed using one of the methods below or collected as chemical waste. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. Ethidium bromide is used to visualize dna in agarose gels and in many cesium chloride gradient protocols.
Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual helix. Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes. Ethidium bromide solution 10 mgml catalog or product number. Ethidium bromide is a toxic chemical and a mutagen. Thats because the compound is used to make dna fluoresce when running gels, and it does that by slipping neatly betw. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and. Dec 19, 20 ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. Ethidium bromide is an intercalating or stacking dye that enters the purine and pyrimidine base pairs. Pubmed comprises more than 26 million citations for biomedical literature from medline, life science journals, and online books. Adding ethidium directly to samples prior to loading. A uv light can be used to determine complete inactivation.
Your new online msds binder is a place for you to store the material safety data sheets. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet. Material safety data sheet section 1 ethidium bromide 1 chemical product and company identification msds name. Do not dispose of ethidium bromide as biohazardous waste. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes. What is the alternative for ethidium bromide in gel electrophoresis. Ethidium bromide safe handling, disposal, and emergency procedures. That said, when mum brought back some veinasbestos samples from. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Exposure information commercial availabili production and producers.
Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as provided for csc1 gradient protocols. Citations may include links to fulltext content from pubmed central and publisher web sites. Ethidium bromide etbr is commonly used as a nonradioactive dna stain to iden. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. Ethidium bromide, 1% solutionmolecular biology revision date 18jan2018 sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical keep product and empty container away from heat and sources of ignition. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes. Ethidium bromide is found in pretty much every molecular biology lab around. Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual.
After inactivation, the solution, devoid of ethidium bromide,can be poured down the drain with copious amounts of water. Wear gloves and a face mask when working with ethidium bromide in powdered form. Liquid ethidium bromide waste can be collected in the same container as the solid waste. The solution can be used to detect both doublestranded and singlestranded dna. Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t. Rna sample loading buffer, with etbr scbt santa cruz. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid. Product name ethidium bromide, 1% solutionmolecular biology cat no. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. Handling when pure ethidium bromide is used, handling should be performed in a fume hood wearing full protection clothing including a lab coat, closedtoe shoes, chemical resistant gloves and chemical safety goggles. Ethidium bromide etbr, a dark red, crystalline, nonvolatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. Eb was reported by watkins 1952 to be prepared from 3. Standard operating procedure template for ethidium bromide.
Rna sample loading buffer, with etbr ethidium bromide, 50gml is formulated for use in rna sample preparation for denaturing formaldehydeagarose gel electrophoresis. Ethidium bromide is typically purchased in powder or solution form and is soluble in water. Ethidium bromide laboratory spill and waste handling. For staining a gel after electrophoresis, dilute a sample of the stock solution to 0. The disposal of laboratory ethidium bromide remains a controversial subject.
It fluorescents a redorange color under ultraviolet uv light and with increased. The detection limit of dna bound to ethidium bromide is 0. It is preferable to use ecofriendly flourescent dyes such as. Ethidium has several experimentally useful properties including binding to nucleic acids. Feb 27, 2020 ethidium bromide is a potent mutagen which has been commonly used as a nucleic acid stain for many years. As ethidium bromide is carcinogenic, there are several biological and environmental problems are associated with it. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm. Ethidium bromide is known as an intercalating agent. Ethidium bromide solution, molecular grade 10mgml, is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. Typically eb is purchased in powder or solution form and is soluble in water. Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair.
Accidental release measures personal precautions wear respiratory protection. Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. Ethidium bromide waste disposal fact sheet environmental. The crystal or powder form is odorless and appears dark red in color. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. Wipe the area with a damp cloth after the work with ethidium bromide powder is complete. Glow dyes have been specifically formulated for nucleic acid. The flat structure of ethidium bromide allows it to intercalate, or insert, between nitrogenous bases of a dna molecule.
Otherwise, aqueous solutions must be deactivated beforedisposal. Material should be handled according to the material safety data sheet msds. Chemical product and company identification section 1. The more common use of the ethidium bromide stain is the detection of dna or rna gel samples. There is an existing crystal structure in which ethidium bromide is complexed with a ribo cpg dinucleotide. While preparing agarose gels why is ethidium bromide added. Ethidium bromide is a potent mutagen used as a nucleic acid stain. The powder is used to make a stock solution of 10 mgml, and the working concentration for gelstaining is 1 gml. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as. Ethidium reemits this energy as yelloworange light centered at 590 nm. Ethidium bromide is typically in powder or solution form and is soluble in water. Please follow the instructions listed in this fact sheet when handling etbr.
The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards. The fluorescence of ethidium monoazide is weak, but the intensity increases 15fold on binding to dna with excitationemiss. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. Bp210 synonyms no information available recommended use laboratory chemicals. Ethidium bromide is not regulated as hazardous waste at low concentrations, but is treated as hazardous waste by many organizations.
It fluoresces a redorange colour under ultraviolet light and with increased fluorescence when bound to doublestranded dna. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. Citations may include links to fulltext content from pubmed central and. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590. I am trying to minimize background while having nice sharp bands for microarray rtpcr validations. Ethidium bromide is strongly mutagenic, causing living cell mutations. Ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. Are other fluorescent tags used instead of ethidium bromide.
Material safety data sheet ethidium bromide solution sdsmsds. Environmental precautions prevent further leakage or spillage if safe to do so. Ethidium bromide solution sc286960 hazard alert code key. Due to its unique structure, it can easily intercalate into dna strand. It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna. Ethidium bromide etbr wastes are not regulated by the state of massachusetts or the epa. A safer alternative is to purchase ethidium bromide in solution. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated dye. Are other fluorescent tags used instead of ethidium. No warranty, express or implied, is made and labchem inc. Ethidium is the fluorescent compound widely used in experimental cell biology and biochemistry to reveal doublestranded dna and rna. Ethidium bromide fact sheet environmental health and safety.
The powder form is considered an irritant to the upper respiratory track, eyes and skin. Ethidium bromide safe handling and disposal procedures. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Adding ethidium directly to samples prior to loading apr052008 hi everyone, ive read somewhere about adding ethidium directly to the samples to be run on gels prior to running. Aqueous buffer solutions containing ethidium bromide can be released down the drain after decontamination or destruction.